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@@ -201,8 +201,9 @@ SDM26 and 9 have similar structures and similar Relative Performances.</li><li>
 SDM3 and SDM5 have a similar structure and a similar Relative Performance.</li></ul>
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+<p> NUPACK analysis also reveals that while calculating the relative performance with respect to cspF, we realised that if a G/C in the original sequence GGAATTTTT was changed to an A/T then the F/OD values went lower. Whereas, if the vice versa was done, the F/OD value increased. We already have an experimentally validated library that gives a range of expression with respect to just the RBS (negative control). Our further designs will be based in learnings and developments from this experiment.<br></p>
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 <p>We wish to modify the GC content of cspF by making multiple mutations in one sequence while maintaining its structural scaffold in the third engineering cycle. The next aspect that we wish to check is that keeping the structural scaffold of the cspF intact we wish to add additional structural complexities of different levels to it through our sequence and see how the gene expression changes.<br></p>
-<p> We have correlated it with NuPACK. However, while calculating the relative performance with respect to cspF, we realised that if a G/C in the original sequence GGAATTTTT was changed to an A/T then the F/OD values went lower. Whereas, if the vice versa was done, the F/OD value increased. We already have an experimentally validated library that gives a range of expression with respect to just the RBS (negative control). Our further designs will be based in learnings and developments from this experiment.<br></p>
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