From 52e8f6543389ed505da0297163fd0a5368711630 Mon Sep 17 00:00:00 2001
From: Arya Ajit Gohad <aryagohad21@gmail.com>
Date: Fri, 16 Dec 2022 18:53:22 +0000
Subject: [PATCH] Update wiki/pages/results.html
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- We have managed to create a library of 5'UTRs that gives a range of expression. The key learning was on how just 9 nucleotides were enough to influence the gene expression as observed in the case of cspF. We are still trying to interpret the complex structures of the naturally occurring UTRs. Our next experimental design shall be more complex based on our understandings and observations of GC content. We further plan to add an additional factor of complexity to our next set of experiments by looking at RNAse cleavage sites. We have realised that experimental results are quite different from software predictions this further justifies the need for more work and attention in the area of 5'UTR. We are completing the learn phase of the second engineering cycle and are contemplating simultaneously over the design of the third as described on our engineering success page. We further wish to develop correlations based on experimentally validated data and build on our 5'UTR Library.</p>
+ We have created a library of 5'UTRs that gives a range of expressions. The key learning was how just nine nucleotides were enough to influence the gene expression, as observed in the case of cspF. We are still trying to interpret the complex structures of the naturally occurring UTRs. Our next experimental design shall be more complex based on our understanding and observations of GC content. We further plan to add a complexity factor to our next set of experiments by looking at RNAse cleavage sites. We have realised that experimental results are quite different from software predictions. This further justifies the need for more work and attention in the area of 5'UTR.
+ </p><p>We conducted our experiments in two types of strains: a cloning strain (DH5alpha) and an expression strain (BL21(DE3)), and obtained expression values with a Spearman's rank correlation coefficient of 0.87. Such a high value indicates that the overall expression trend remains the same across these two strains. We also wish to conduct these experiments in wild-type strains like MG1655 and other types of strains so that the data we generate and contribute to the Library is expansive and useful to as many researchers as possible. We further wish to develop correlations based on experimentally validated data and build on our 5'UTR Library. We have completed the second engineering cycle and are now contemplating the design of the third, as described on our engineering cycle pages. </p>
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<br><center><img class="norm" src="https://static.igem.wiki/teams/4303/wiki/bl-all-utrs.jpg" alt="Image"><center>
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