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BBrickIt is a software that BioBricks any DNA sequence for you. According to the BioBrick standards, each BioBrick part must have a Prefix and Suffix. In order for a part to be compatible with BioBrick RFC[10] standards, it must not contain the following restriction sites, as these are unique to the prefix and suffix:
Sequence | Type | Enzyme |
---|---|---|
gaattc | Illegal | EcoRIt |
tctaga | Illegal | XbaI |
actagt | Illegal | SpeI |
ctgcag | Illegal | PstI |
gcggccgc | Avoid | NotI |
BBrickIt v.2 builds upon the previous version of BBrickIt. This previous version of BBrickIt has been completely developed by our own ICT Mumbai iGEM Team of 2018. We have modified it this year. Users can now input an mRNA sequence in the input field and the software will convert it to the corresponding DNA sequence and return the BioBrick. It also comes with a “Copy BioBrick Sequence” button to enable users to copy the BioBrick sequence to their clipboard with the click of a button. Several bug fixes have also been made.
Steps to Use BBrickIt:
You can find the source code and executable file here.