From 01c70b6266d14bd1816ed87157f827a1dac1dbb9 Mon Sep 17 00:00:00 2001
From: Kong Yangyang <kimmy.kong@cataverse.cn>
Date: Fri, 26 Aug 2022 07:52:40 +0000
Subject: [PATCH] Update wiki/pages/description.html
---
wiki/pages/description.html | 123 +++++++++++++++++++++---------------
1 file changed, 73 insertions(+), 50 deletions(-)
diff --git a/wiki/pages/description.html b/wiki/pages/description.html
index 9027e2a..bfefeab 100644
--- a/wiki/pages/description.html
+++ b/wiki/pages/description.html
@@ -1,57 +1,80 @@
{% extends "layout.html" %}
-
-{% block title %}Project Description{% endblock %}
-{% block lead %}Describe how and why you chose your iGEM project.{% endblock %}
-{% block page_content %}
-
-<div class="row mt-4">
- <div class="col">
- <div class="bd-callout bd-callout-info">
- <h4>Bronze Medal Criterion #3</h4>
- <p>Describe how and why you chose your iGEM project.<p>
- <hr>
- <p>Please see the <a href="https://competition.igem.org/judging/medals">2022 Medals Page</a> for more information.</p>
- </div>
- </div>
-</div>
+{% block title %}Description{% endblock %}
-<div class="row mt-4">
- <div class="col-lg-8">
- <h2>What should this page contain?</h2>
- <hr>
- <ul>
- <li>A clear and concise description of your project.</li>
- <li>A detailed explanation of why your team chose to work on this particular project.</li>
- <li>References and sources to document your research.</li>
- <li>Use illustrations and other visual resources to explain your project.</li>
- </ul>
- </div>
- <div class="col-lg-4">
- <h2>Inspirations</h2>
- <hr>
- <ul>
- <li><a href="https://2019.igem.org/Team:Leiden/Description">2019 Leiden</a></li>
- <li><a href="https://2019.igem.org/Team:ITESO_Guadalajara/Description">2019 ITESO Guadalajara</a></li>
- <li><a href="https://2020.igem.org/Team:Technion-Israel/Description">2020 Technion Israel</a></li>
- <li><a href="https://2020.igem.org/Team:Botchan_Lab_Tokyo/Description">2020 Botchan Lab Tokyo</a></li>
- <li><a href="https://2020.igem.org/Team:St_Andrews/Description">2020 St Andrews</a></li>
- <li><a href="https://2020.igem.org/Team:MIT/Description">2020 MIT</a></li>
- </ul>
- </div>
-</div>
+{% block header_content %}
+<img class="sub-header-logo" src="https://static.igem.wiki/teams/4515/wiki/banner.jpg" />
+{% endblock %}
-<div class="row mt-4">
- <div class="col-lg-8">
- <h2>Some advice</h2>
- <hr>
- <p>We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements. Your Project Description should include more information than your project abstract.</p>
- </div>
- <div class="col-lg-4">
- <h2>References</h2>
- <hr>
- <p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
+{% block page_content %}
+<div class="sub-page-bg">
+ <div class="sub-page-content">
+ <div class="content-title">Description</div>
+ <div class="title blue-title">1. Background</div>
+ <div class="article-content ">
+ N-butanol, an important chemical raw material, is expected to become one of the new generations of biofuels. At
+ present, the domestic industrial synthesis of N-butanol mainly adopts the low-pressure carbonyl synthesis method,
+ from the production process, propylene, CO, H2, and carbonyl are the main production materials. However, this
+ production method relies on non-renewable petroleum products as essential raw materials, which is not friendly to
+ the environment. Therefore, it is necessary to design an environmentally friendly production method for N-butanol
+ to meet the demand of environmental protection. At present, biosynthesis of N-butanol is an important research
+ field of butanol factory production. It has been found that N-butanol can be synthesized naturally in Clostridium,
+ but the tolerance of Clostridium bacteria is not good enough for large-scale production. Recently, Lactobacillus
+ Brevis with better N-butanol tolerance has been isolated by researchers. This project will build an
+ N-butanol-producing bacterium to meet the needs of industrial production and lay a foundation for subsequent
+ improvement.
+ </div>
+ <div class="title blue-title">2. Experiment Design</div>
+ <div class="sub-title">a) The metabolic pathway of N-butanol synthesis in Lactobacillus Brevis ATCC367.</div>
+ <div class="article-content ">Genes introduced into L. brevis are shown in red (Figure 1). The thl gene encodes
+ mercaptan, hbd encodes β -hydroxybutyrate CoA dehydrogenase, crt encodes 3-hydroxybutyrate CoA dehydrase, and ter
+ encodes trans-enol CoA reductase. Only when the four enzymes are expressed together can the engineering strain
+ achieve the metabolic process from glucose to N-butanol.</div>
+ <div class="img-wrap no-margin">
+ <img class="w-40" src="https://static.igem.wiki/teams/4515/wiki/t-east-china-description01.jpg" />
+ <span>Figure 1. The metabolic pathway of N-butanol synthesis in Lactobacillus Brevis ATCC367. </span>
+ </div>
+ <div class="sub-title">General Experiment Procedure</div>
+ <div class="article-content ">
+ First, the N-butanol fermentation-related genes, thlA, hbd, crt, and ter genes were amplified by PCR from the
+ Lactobacillus Brevis ATCC824 genomic DNA, and then amplicons were extracted.<br />
+ Next, we extracted plasmids pIB184-vector from E. coli DH5α, ligated the target gene and the vector into complete
+ plasmids with homologous recombinant enzymes, and transferred the recombinant plasmids into Streptococcus Brevis
+ by electroporation. <br />
+ Then, erythromycin was used to select whether the plasmid was inserted into the competent bacteria Streptococcus
+ Brevis ATCC367 and screened, and the screened bacteria were coated on MRS solid medium dish. If there are positive
+ colonies, we cultivate them in the liquid medium and incubate them in the anaerobic chamber. <br />
+ Finally, when our bacteria grew, we measured the growth curve and detected the yield of N-Butanol (Figure 2).
+ </div>
+ <div class="img-wrap no-margin">
+ <img class="w-60" src="https://static.igem.wiki/teams/4515/wiki/t-east-china-description02.png" />
+ <span>Figure 2. General experiment procedure</span>
+ </div>
+ <div class="title blue-title">3. Expected Result</div>
+ <div class="article-content">
+ 1. Successfully construct thlA, hbd, crt, and ter genes into double-enzyme-digested vector pIB184.<br />
+ 2. Transform the recombinant plasmid into Streptococcus Brevis ATCC367 and identify the positive colony.<br />
+ 3. Detect the yield of N-Butanol and measure the growth curve of the engineered strain.<br />
+ The study shows that Streptococcus Brevis has a high tolerance of N-butanol and is likely to be a candidate for
+ producing high concentrations of N-butanol.
+ </div>
+ <div class="title blue-title">4. Reference</div>
+ <div class="article-content">
+ 1. å¼ äº‘è´¤, å¼ åŽè¥¿, 余维新, æŽæ°çµ, & è°å¹³åŽ. (2015). æ£ä¸é†‡çš„åˆæˆè¿›å±•ç®€è¿°. 2015 ä¸å›½åŒ–å·¥å¦ä¼šå¦æœ¯å¹´ä¼š. <br />
+ 2. Li, J., Zhao, J. B., Zhao, M., Yang, Y. L., Jiang, W. H., & Yang, S. (2010). Screening and characterization of
+ butanol-tolerant micro-organisms. Letters in applied microbiology, 50(4), 373–379.
+ https://doi.org/10.1111/j.1472-765X.2010.02808.x <br />
+ 3. Berezina, O. V., Zakharova, N. V., Brandt, A., Yarotsky, S. V., Schwarz, W. H., & Zverlov, V. V. (2010).
+ Reconstructing the clostridial n-butanol metabolic pathway in Lactobacillus brevis. Applied microbiology and
+ biotechnology, 87(2), 635–646. https://doi.org/10.1007/s00253-010-2480-z <br />
+ 4. Inui M, Suda M, Kimura S, Yasuda K, Suzuki H, Toda H, Yamamoto S, Okino S, Suzuki N, Yukawa H (2008) Expression
+ of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli. Appl Microbiol Biot 77:1305–1316.
+ https://doi.org/10.1007/s00253-007-1257-5 <br />
+ 5. Mitchell WJ (1998) Physiology of carbohydrate to solvent conversion by Clostridia. In: Poole RK (ed) Advances
+ in Microbial Physiology, vol 39. pp 31–130 <br />
+ 6. Bowles LK, Ellefson WL (1985) Effects of butanol on Clostridium-acetobutylicum. Appl Environ Microb
+ 50:1165–1170
+ </div>
</div>
</div>
-
{% endblock %}
--
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