{% extends "layout.html" %} {% block title %}Human Practices{% endblock %} {% block lead %}We ask every team to think deeply and creatively about whether their project is responsible and good for the world. Consider how the world affects your work and how your work affects the world.{% endblock %} {% block page_content %}
We have built 5 new Basic Parts and 5 Composite Parts in total. Five Composite Parts are used to express CA,CA2,PRK,CA-PRK and CA2-PRK respectively.
Description | Type | Name |
CYC1 | Terminator | BBa_K3812006 |
BW25113 | RBS | BBa_K3812011 |
6xHis-tag | Tag | BBa_K3812012 |
Paox1 | Promoter | BBa_K3812005 |
pSB1C3 | Plasmid | BBa_K3812014 |
CA2 | Coding | BBa_K4112005 |
CA | Coding | BBa_K4112006 |
PRK | Coding | BBa_K4112007 |
CA2-PRK | Coding | BBa_K4112008 |
CA-PRK | Coding | BBa_K4112009 |
Description | Type | Name |
CA2 | Composite | BBa_K4112010 |
CA | Composite | BBa_K4112011 |
PRK | Composite | BBa_K4112012 |
CA2-PRK | Composite | BBa_K4112013 |
CA-PRK | Composite | BBa_K4112014 |
The PTCA-encoded carbonic anhydrase of P. tricornutum was cloned by overlap PCR. As a member of the β-CA family, it can reversibly catalyze the CO2 hydration reaction, which can improve CO2 conversion efficiency and play a key role in CCM, as well as in ion exchange, CO2 acquisition, and photosynthesis.
PTCA2, which is highly homologous to PTCA, encodes a protein with fully conserved β-CA zinc coordination residues and significant levels of mRNA accumulation at low CO2 concentrations.
PRK encodes phospho-ribulose kinase, an enzyme specific to the Calvin cycle, which plays a crucial role in regulating the flow of sugars in the Calvin cycle by catalyzing the formation of the receptor ribulose-1,5-bisphosphate of CO2 with the help of ATP phosphorylation.
Use linker to connect them separately, build CA-PRK and CA2-PRK, transferred to E. coli and tested.