diff --git a/wiki/pages/engineering.html b/wiki/pages/engineering.html index 3d6ab36977592f41d17921704807168452e5531c..bd277791c8a8d7a4abf985ce849088ce31eda8de 100644 --- a/wiki/pages/engineering.html +++ b/wiki/pages/engineering.html @@ -284,60 +284,42 @@ <div class="grid-content right-short"> <h1>Learn</h1> <p> - Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aliquam venenatis ex nibh, quis - imperdiet - lectus hendrerit vitae. Nulla non ex suscipit, fringilla enim gravida, mollis ante. Nullam - vel - vestibulum orci. Curabitur id ex ligula. Suspendisse maximus arcu sagittis, ultricies sapien - at, - posuere sem. Cras vel dolor lobortis, vehicula metus nec, ullamcorper diam. Sed pretium nibh - lectus, - id euismod urna consectetur non. Nulla facilisi. Praesent congue magna ac neque lobortis, - vel - sagittis neque euismod. Donec venenatis augue quis lorem fermentum, id dapibus nunc - pharetra. - Praesent aliquam gravida lacus, sit amet rhoncus nunc dignissim quis.<br/><br/> - - Quisque rhoncus libero quis odio ornare maximus. Vivamus quis condimentum massa. In luctus - hendrerit - dolor, quis blandit nisi porta accumsan. Nullam neque sapien, molestie non ex convallis, - ornare - consectetur ante. Integer a, <strong>gravida enim sed</strong>, molestie sem. Nam tincidunt - sapien - et - justo cursus, ut placerat sem sagittis. Cras maximus purus augue, ut cursus nunc - pellentesque - ut. - Fusce commodo libero et egestas rutrum. Cras ac justo lorem. Maecenas tempus ut leo sit amet - rutrum.<br/><br/> - - Morbi blandit cursus diam id ultrices. Vestibulum a vulputate justo. Quisque volutpat - finibus - efficitur. Ut ultricies nibh non arcu suscipit dapibus. Phasellus interdum bibendum nibh sed - tincidunt. Pellentesque in felis metus. Phasellus euismod consequat dignissim. Proin in - efficitur - tortor. Quisque sagittis facilisis tortor nec commodo. Integer condimentum sagittis luctus. - - Phasellus congue rhoncus eros, euismod efficitur neque. Etiam sagittis vitae ligula non - iaculis. - In - volutpat sollicitudin risus nec venenatis. Donec elementum, sem eu ornare bibendum, enim - tortor - cursus mauris, sit amet dignissim risus ante vel justo. Sed fermentum erat id nibh interdum - sollicitudin. Vestibulum tempor metus ac libero maximus semper. Donec sed ligula neque. - Morbi - sagittis orci odio, eget blandit urna molestie hendrerit. Fusce orci tellus, iaculis at - pretium - et, - convallis in tortor. Curabitur finibus orci tortor, eget euismod lectus imperdiet quis.</p> + Through our tests, we were able to learn many things about our aptamer probe. For example, we learned that + the aptamer probes had much weaker fluorescence values than we had imagined. With a 100µL solution of 500µM + aptamer solution and 500µM biomarker solution, the maximum Relative Fluorescence Unit (RFU) was 846. While + this is a measurable value, we realized that there is much more research needed to improve this system and + increase the RFU value in some way. This would be able to prevent false positives during screenings, and we + would recommend other teams to explore this topic of amplification.<br><br> + Furthermore, we were able to verify the specificity and durability of aptamers. Although we did know through + literature that aptamers were better in terms of specificity and durability compared to antibodies, we were + able to verify this through our specificity test and shelf life test. Our experiments build off of prior + experiments with aptamers, and is another example of the versatility of aptamers probes.<br><br> + We also learned that the FRET mechanism works very well. In our experiments, when we had only a solution of + S2.2 aptamer probes without Mucin 1 biomarkers, the aptamer probes virtually did not fluoresce, since the + RFU values were similar to that of pure buffer solution. This exemplifies the high accuracy of the aptamer + probe mechanism utilizing the FRET mechanism to make the aptamer probes only fluoresce in the presence of + the desired target. + </p> </div> <div class="grid-gap"></div> <div class="grid-gap"></div> <div class="grid-content left-short"> <h1>Design</h1> <p> - - + We explored various previous literature conducted on breast cancer patients, as well as literature on the + detection of breast cancer through the ELISA method detecting levels of Mucin 1 biomarker. As shown in the + box and whisker chart below, literature showed that the error bars of the standard mean error overlapped + between breast cancer patients and healthy patients. + <div style="display: flex; flex-direction: column; align-items: center; width: 100%; margin: 14px 0"> + <img width="50%" src="https://static.igem.wiki/teams/4334/wiki/engineeringchart.png"> + <div class="smaller">(Werfalli, et al.)</div> + </div> + Thus, we determined that our method may not provide the most accurate assessment of who requires further + screening due to risk of false positives—and alarming potential patients is a situation we’d like to avoid. For + this reason, we propose to implement our screening method to detect diseases where any presence of biomarkers is + an indication for the necessity of treatment (including hepatitis B virus). When it comes to breast cancer + applications, we propose to apply this method in the future where technology for the identification of Mucin 1 + biomarker concentrations in patients with breast cancer have improved. </p> </div> <div class="grid-gap"></div>